Colorimetric detection of norovirus by helicase-dependent amplification method based on specific primers integrated with HRPzyme
- Lee, Jeong-Eun; Kim, Sol-A; Park, Hyun-Jin; Mun, Hyoyoung; Ha, Kwang-Soo; Shim, Won-Bo
- Issue Date
- SPRINGER HEIDELBERG
- Norovirus (NoV); Helicase-dependent amplification; Colorimetric detection; HRPzyme
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.414, no.23, pp.6723 - 6733
- Journal Title
- ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Start Page
- End Page
- Noroviruses (NoVs) are the most common causes of epidemic gastroenteritis, responsible for at least 50% of all gastroenteritis outbreaks worldwide and significant causes of foodborne illness. In the USA, approximately 21 million illnesses attributable to NoVs have annually occurred. Therefore, there is a great demand to develop a rapid, low-cost, and accurate detection method for NoVs. This study first reported colorimetric helicase-dependent amplification (HDA) methods based on specific primers integrated with HRPzyme for the rapid and sensitive detection of NoV GI and GII. The colorimetric HDA methods exhibited a detection limit of 10 copies mL(-1) of each NoV GI and GII and were confirmed to be specific to each NoV GI and GII. The period required to complete the HDA method was 2 h, including a step of RNA extraction and cDNA synthesis without expensive instruments such as a thermal cycler and detector. The cutoff value of the method for the oyster artificially inoculated with a known amount of NoV was all 10(2) copies g(-1) for NoV GI and GII. Therefore, the HDA method developed in this study can be useful tool for the on-site detection of NoVs in food samples.
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