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Cited 43 time in webofscience Cited 46 time in scopus
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A Chronic Immobilization Stress Protocol for Inducing Depression-Like Behavior in Mice

Authors
Son, HyeonwiYang, Ju HwanKim, Hyun JoonLee, Dong Kun
Issue Date
May-2019
Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
Keywords
Behavior; Issue 147; neuroscience; animal model; depression; chronic immobilization stress; tail suspension test; sucrose preference test; corticosterone; C57BL/6 strain
Citation
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v.2019, no.147
Indexed
SCIE
SCOPUS
Journal Title
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume
2019
Number
147
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/9184
DOI
10.3791/59546
ISSN
1940-087X
Abstract
Depression is not yet fully understood, but various causative factors have been reported. Recently, the prevalence of depression has increased. However, therapeutic treatments for depression or research on depression is scarce. Thus, in the present paper, we propose a mouse model of depression induced by movement restriction. Chronic mild stress (CMS) is a well-known technique to induce depressive-like behavior. However, it necessitates a complex procedure consisting of a combination of various mild stresses. In contrast, chronic immobilization stress (CIS) is a readily accessible chronic stress model, modified from a restraint model that induces depressive behavior by restricting movement using a restrainer for a certain period. To evaluate the depressive-like behaviors, the sucrose preference test (SPT), the tail suspension test (TST), and the ELISA assay to measure stress marker corticosterone levels are combined in the present experiment. The described protocols illustrate the induction of CIS and evaluation of the changes in behavior and physiological factors for the validation of depression.
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의과대학 (의학과)
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