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Loop-mediated isothermal amplification of PBAN gene for molecular diagnosis of Bemisia tabaci biotype Q (Hemiptera: Aleyrodidae)Loop-mediated isothermal amplification of PBAN gene for molecular diagnosis of Bemisia tabaci biotype Q (Hemiptera: Aleyrodidae)

Other Titles
Loop-mediated isothermal amplification of PBAN gene for molecular diagnosis of Bemisia tabaci biotype Q (Hemiptera: Aleyrodidae)
Authors
Lee, JunbeomLee, Byoung-heePark, Jung-JoonJeong, In HongLee, Dae-Weon
Issue Date
Sep-2022
Publisher
KOREAN SOC APPLIED ENTOMOLOGY
Keywords
Bemisia tabaci; PBAN; LAMP; biotype Q; diagnostic PCR
Citation
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, v.25, no.3, pp 1 - 4
Pages
4
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
Volume
25
Number
3
Start Page
1
End Page
4
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/917
DOI
10.1016/j.aspen.2022.101942
ISSN
1226-8615
1876-7990
Abstract
The tobacco whitefly, Bemisia tabaci (Hemiptera: Aleymdidae), is a serious pest that transmits tomato yellow leaf curl virus (TYLCV) in tomato. The tobacco whitefly exhibits morphological similarity with the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) which also damages various plants in the greenhouse. Therefore, it is very important to quickly and accurately diagnose whiteflies for applying pest management strategies. In this study, we used a loop-mediated isothermal amplification (LAMP) assay to quickly and effectively detect B. tabaci. Primer sets were investigated for the specificity of B. tabaci by visible detection of the target DNA fragment. Two primer sets for pheromone biosynthesis activating neuropeptide (PBAN) gene were obtained from transcriptomic analysis of B. tabaci biotype Q. The PBAN-based LAMP primer set showed specific amplification of the target region. The optimal conditions for B. tabaci detection were 60 degrees C for 60 min with four LAMP primers of PBAN. The minimum amount of genomic DNA required for visible detection was 100 ng. These results suggest that the PBAN-based LAMP assay can be applicable for field monitoring of B. tabaci.
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농업생명과학대학 (식물의학과)
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