One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencheropen access
- Kim, Hong-Rae; Bong, Ji-Hong; Kim, Tae-Hun; Shin, Seung-Shick; Kang, Min-Jung; Shim, Won-Bo; Lee, Do Young; Son, Dong Hee; Pyun, Jae-Chul
- Issue Date
- KOREAN BIOCHIP SOCIETY-KBCS
- One-step homogeneous immunoassay; Switching peptide; Influenza-A and influenza-B virus; Lateral flow immunoassay
- BIOCHIP JOURNAL, v.16, no.3, pp.334 - 341
- Journal Title
- BIOCHIP JOURNAL
- Start Page
- End Page
- One-step homogeneous immunoassay was developed for detecting influenza viruses A and B (Inf-A and Inf-B) using the switching peptide H2. As the fluorescence-labeled switching peptide dissociated from the binding pocket of detection antibodies, the fluorescence signal could be directly generated by the binding of Inf-A and Inf-B without washing (i.e., one-step immunoassay). For the one-step homogeneous immunoassay with detection antibodies in solution, graphene was labeled with the antibodies as a fluorescence quencher. To test the feasibility of the homogeneous one-step immunoassay, the stability of the antibody complex with the switching peptide was evaluated under different pH and salt conditions. The one-step homogeneous immunoassay with switching peptide was conducted using influenza virus antigens in phosphate-buffered saline and real samples with inactivated Inf-A and Inf-B spiked in serum. Finally, the one-step homogeneous immunoassay results were compared with those of commercially available lateral flow immunoassays.
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- 농업생명과학대학 > 식품공학부 > Journal Articles
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