Detailed Information

Cited 1 time in webofscience Cited 2 time in scopus
Metadata Downloads

Identification of changed proteins by retinoic acid in cerebral ischemic damage: a proteomic studyopen access

Authors
Kang, J.-B.Koh, P.-O.
Issue Date
Sep-2022
Publisher
Maruzen Co., Ltd/Maruzen Kabushikikaisha
Keywords
cerebral ischemia; neuroprotection; proteomics; retinoic acid
Citation
Journal of Veterinary Medical Science, v.84, no.9, pp 1194 - 1204
Pages
11
Indexed
SCIE
SCOPUS
Journal Title
Journal of Veterinary Medical Science
Volume
84
Number
9
Start Page
1194
End Page
1204
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/897
DOI
10.1292/jvms.22-0119
ISSN
0916-7250
1347-7439
Abstract
Ischemic stroke is a severe neurodegenerative disease with a high mortality rate. Retinoic acid is a representative metabolite of vitamin A. It has many beneficial effects including anti-inflammatory, anti-apoptotic, and neuroprotective effects. The purpose of this study is to identify specific proteins that are regulated by retinoic acid in ischemic stroke. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Retinoic acid (5 mg/ kg) or vehicle was injected intraperitoneally into male rats for four days prior to MCAO operation. Neurobehavioral tests were performed 24 hr after MCAO and the cerebral cortex was collected for proteomic study. Retinoic acid alleviates neurobehavioral deficits and histopathological changes caused by MCAO. Furthermore, we identified various proteins that were altered by retinoic acid in MCAO damage. Among these identified proteins, adenosylhomocysteinase, isocitrate dehydrogenase [NAD+ ] subunit α, glycerol-3-phosphate dehydrogenase, Rab GDP dissociation inhibitor β, and apolipoprotein A1 were down-regulated in MCAO animals with vehicle treatment, whereas retinoic acid treatment alleviated these reductions. However, heat shock protein 60 was up-regulated in MCAO animals with vehicle, while retinoic acid treatment attenuated this increase. The changes in these expressions were confirmed by reverse transcription-PCR. These proteins regulate cell metabolism and mediate stress responses. Our results demonstrated that retinoic acid attenuates the neuronal damage by MCAO and regulates the various protein expressions that are involved in the survival of cells. Thus, we can suggest that retinoic acid exerts neuroprotective effects on focal cerebral ischemia by modulation of specific proteins. ? 2022 The Japanese Society of Veterinary Science.
Files in This Item
There are no files associated with this item.
Appears in
Collections
수의과대학 > Department of Veterinary Medicine > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Koh, Phil Ok photo

Koh, Phil Ok
수의과대학 (수의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE