Upregulated TTYH2 expression is critical for the invasion and migration of U2OS human osteosarcoma cell lines
- Moon, Dong Kyu; Bae, Yeon Ju; Jeong, Geuk-Rae; Cho, Chang-Hoon; Hwang, Sun Chul
- Issue Date
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Osteosarcoma; TTYH2; Invasion; Migration; Proliferation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.516, no.2, pp.521 - 525
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Start Page
- End Page
- Ion channels have recently emerged as stable biomarkers and anticancer targets particularly when the applications of the currently available therapeutic regimens are limited, as in case of osteosarcoma, a malignant bone tumor. Here, we evaluated the expression of TTYH2, a presumably calcium-activated chloride channel, in a human osteosarcoma cell line U2OS. We used small-interfering RNA (siRNA)-mediated gene silencing to demonstrate the downregulation in the expression of TTYH2 that resulted in the decrease in the invasion and migration, but not proliferation, of U2OS cells. The expression levels of Slug and ZEBI, the transcription factors involved in epithelial-mesenchymal transition, significantly reduced after TTYH2 silencing. Based on these results, we suggest that TTYH2 may serve as a novel target for the treatment of osteosarcoma. (C) 2019 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
- College of Medicine > Department of Medicine > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.