Chemokine receptor 4 (CXCR4) blockade enhances resistance to bacterial internalization in RAW264.7 cells and AMD3100, a CXCR4 antagonist, attenuates susceptibility to Brucella abortus 544 infection in a murine model
- Reyes, Alisha Wehdnesday Bernardo; Arayan, Lauren Togonon; Tran Xuan Ngoc Huy; Vu, Son Hai; Kang, Chang Keun; Min, Wongi; Lee, Hu Jang; Lee, John Hwa; Kim, Suk
- Issue Date
- AMD3100; B. abortus; Cytokines; CXCR4; Invasion; MAPKs
- VETERINARY MICROBIOLOGY, v.237
- Journal Title
- VETERINARY MICROBIOLOGY
- We investigated the involvement of chemokine receptor type 4 (CXCR4) signaling on the outcome of Brucella (B.) abortus 544 infection in murine macrophages and in a mouse model. CXCR4 manipulation were first evaluated for Brucella invasion and intracellular survival efficiency, mitogen-activated protein kinases (ERK1/2, JNK, p38a) activation and generation of nitric oxide (NO), and then in the splenic bacterial proliferation and cytokine production in BALB/c mice. CXCR4 blockade is involved in the successful control of Brucella invasion, reduction of ERK1/2 phosphorylation and inhibition of nitric oxide release from macrophages. Furthermore, using a reported CXCR4-specific antagonist AMD3100 resulted in splenomegaly but attenuated Brucella proliferation in these organs with elevated serum levels of MCP-1, TNF and IL-12. These findings provide insights on the contribution of CXCR4 signaling in the phagocytic pathway and immune modulation during B. abortus infection.
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- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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