한방사료 첨가제의 항균성 및 재래닭에서의 Campylobacter jejuni 방제효과

Abstract

In this study, antimicrobial activity of oriental herbal medicine extract (OHME) was tested for someorganisms and the preventive effects of OHME for the colonization of Campylobacter jejuni on epithelium of smallintestine were examined in Korean native broiler chickens fed a forage added 1.0% OHME. The isolated Campylobactersp were biotyped, serotyped and the susceptiblility of isolates to antimicrobial agent were examined. The growthof Staphylococcus aureus was inhibited in 0.25% OHME. C. jejuni and C. coli were inhibited in 0.1% OHME, andSalmonella spp, Lactobacillus acidophilus and Escheichia coli 0157 were inhibited in 2.0% OHME. For the applicationof forage added 1.0% OHME in broiler chicken farm, the frequency of Campylobacter spp from feces, liver and spleensample of chickens were examined during 2 weeks interval. The frequence of Campylobacter spp in feces from chickensof Campylobacter spp in feces sample from chickens fed forage added OHME was slightly reduced from 25% infirst week to 15% in seventh week. The frequence of Campylobacter spp in liver, and spleen was 13.7% and 10%respectively after seventh week in control group, but the Campylobacter spp was not isolated after fifth week in liveand spleen from chickens fed forage added OHME. Isolated 56 strains of thermophilic Campylobacter from Koreannative chickens was classified as C. jejuni (76.7%), C. coli (214%) and C. laridis (1.6%). The majority of 43 isolatesof C. jejuni was clasified on biotype I (60.4%), I (30.2%). Most of 12 isolates of C.coli were biotype I (83.3%).Isolated 31strains C. jejuni of showed 11 diferent serotype, and serotype 36 (18.6%), 17 (13.9%)were most frequent.Isolated 10 strains of C. coli showed 5 different serotypes and serotype 31 (33.3%) and 21 (25%) were relativelyCampylobacter sp were highly susceptible to nalidixic acid, amikacin, gentamicin, colistin andchloramphenucol.