White Leghorn에서 산란 단계에 따른 소장의 소화 효소 및 소장, 간 및 난관 조직의 항산화 작용

Abstract

The study aimed to investigate changes in intestinal hydrolases and antioxidant parameters in the digestive and reproductive organs of White Leghorn at different laying stages: 18-weeks (initial laying stage, ILS), 36-weeks (peak laying stage, PLS), and 62-weeks (late laying stage, LLS). Body weight of hens significantly increased from 18- to 36-weeks (P<0.05), but remained unchanged from 36- to 62-weeks. The relative weights (g/100 g of BW) of the intestinal mucosa and spleen decreased as the laying stages progressed (P<0.05). In contrast, the relative weight of the oviduct was significantly higher in hens at 36- and 62-weeks compared to those at 18-weeks (P<0.05). The specific activity of intestinal maltase and alkaline phosphatase was significantly higher at 18-weeks compared with 62-weeks layers (P<0.05), while the activities of sucrase and leucine aminopeptidase remained unchanged. In the antioxidant parameters, superoxide dismutase (SOD) activity in the intestinal mucosa was at the lowest level at 62-weeks compared with those of 18-weeks (P<0.05). Moreover, SOD, glutathione peroxidase, and catalase activity in the liver and oviduct significantly decreased at 62-weeks compared to 18- or 36-weeks (P<0.05). However, glutathione S-transferase activity and malondialdehyde in the liver significantly increased as the laying stage progressed, resulting in the highest level at 62-weeks (P<0.05). In conclusion, there was a significant laying stage-related change in intestinal hydrolase and antioxidant enzymes in the intestine, liver, and oviduct. This results suggest that the LLS hens were exposed to considerable oxidative stress in the digestive and reproductive organs compared with the ILS and PLS hens.