Lacrimispora sanguinis sp. nov., isolated from human blood

Abstract

A rod-shaped, obligate anaerobic, Gram-stain-positive bacterium isolated from the human blood was designated as the strain HJ-01T. Analysis of the 16S rRNA gene sequence revealed that the strain HJ-01T belonged to the genus Lacrimispora, and was most closely related to L. celerecrescens strains DSM 105336 and MCM B-936, with both 99.3% similarity. The average nucleotide identity values between the strain and the most closely related type strains ranged from 75.3% to 91.4%, while the values between the strain and the two non-type strains of L. celerecrescens, DSM 105336 and MCM B-936, were 98.8% to 98.9%. The digital DNA-DNA hybridization values between the strain and the most closely related type strains ranged from 19.8% to 44.5%, whereas the values between the strain and L. celerecrescens strains DSM 105336 and MCM B-936 were 89.7% to 91.6%. The phylogenomic analysis revealed that the strain formed a cluster adjacent to L. celerecrescens strains DSM 105336 and MCM B-936. The main fatty acids identified were C16:0 and C18:1 cis 11 DMA. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. The end products of the fermentation were acetic acid and formic acid. The strain HJ-01T and the related Lacrimispora strains shared similar antibiotic resistance profiles, including high resistance to clindamycin (8-256 mu g ml-1), linked to the cfr(C) gene located within a 3,378-bp chromosomal transposed unit. Given the chemotaxonomic, phenotypic, and phylogenetic properties, HJ-01T (= KCTC 25933T = JCM 37550T) represent a novel species of the genus Lacrimispora, for which the name Lacrimispora sanguinis sp. nov. is proposed. Additionally, we suggest that L. celerecrescens DSM 105336 and MCM B-936 be transferred to Lacrimispora sanguinis sp. nov.