HT-29와 HT22 세포에서 H2O2 및 LPS로 유도된 산화 스트레스에 대한 머위 잎 추출물의 보호 효과

Abstract

This study investigated the antioxidant activities and neuroprotective effects of 20% ethanolic extract of Petasites japonicus leaves (EPJ), focusing on its potential to enhance probiotic growth. EPJ exhibited significant antioxidant activity, as determined by 2,2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging assays, along with its inhibitory effect on malondialdehyde (MDA) formation. EPJ efficiently inhibited α-glucosidase and acetylcholinesterase. EPJ also protected HT22 and HT-29 cells against H2O2 and LPS-induced cytotoxicity, increasing cell viability by inhibiting reactive oxygen species (ROS) production. EPJ also exerted an appreciable effect on the viability and titratable acidity of Levilactobacillus brevis and Limosilactobacillus reuteri. EPJ’s bioactive compounds were identified as chlorogenic acid and ferulic acid using high-performance liquid chromatography (HPLC). These results suggest that EPJ is a potential functional food source for improving intestinal health and ameliorating neurodegenerative diseases.