Effect of Seed Hydropriming on the Elongation of Plumule and Radicle During the Germination Process and Changes in Enzyme Activity Under Water-Deficient Conditions

Abstract

Hydropriming rice seeds effectively improve the germination percentage, shortens the germination period, and promotes seedling growth. The impact of seed hydropriming is to speed up growth under dry soil conditions, thereby avoiding drought damage. This study analyzes the effect of hydropriming on morpho-physiological changes in the water uptake of rice seeds using "Kasalath" and "Nipponbare" under water-deficit conditions. Upon exposure to osmotic stress, both varieties showed delays in the time to reach germination. In addition, all germination phases exhibited reductions in the activity of alpha-amylase and total soluble sugar by osmotic stress; however, in all germination phases of the hydroprimed seeds, the activity and contents of those were significantly increased, resulting in increased size of the coleoptile, plumule, and radicle. In hydroprimed seeds, "Kasalath" was superior to "Nipponbare" in the ratio of the water-deficit-to-well-watered conditions for all traits related to germination, which may have been attributable to hydropriming having a greater effect on "Kasalath". Interestingly, Primed "Kasalath" had a lower level of alpha-amylase, despite the having a higher content of total soluble sugars than primed "Nipponbare".