Biokinetics of fluorophore-conjugated polystyrene microplastics in marine mussels

Abstract

Biokinetic information on microplastics in bivalves is required to reduce the human exposure, but little is known about the time-course and size effect on tissue absorption and clearance. The biokinetics of fluorophore-labeled polystyrene microbeads with diameters 10 mu m (PL10) and 90 mu m (PL90) in Mytilus galloprovincialis marine mussels was investigated in the present study. It was found that both PL10 and PL90 showed a biphasic tissue distribution pattern in digestive and non-digestive tissues, highlighting the significant tissue distribution starting from 48 h post-treatment. The differential size effect on tissue distribution was observed only in the gills, which suggests that PL10 accumulates more than PL90. The depuration kinetics show that particles of both sizes can be cleared in any tissue, but non-digestive tissue requires a longer duration for depuration than digestive tissue. The differential size effect on depuration was observed for both digestive and non-digestive tissues, suggesting that