Application of proteomics for comparison of proteome of Neospora caninum and Toxoplasma gondii tachyzoites
- Authors
- Lee, E.-G.; Kim, J.-H.; Shin, Y.-S.; Shin, G.-W.; Kim, Y.-R.; Palaksha, K.J.; Kim, D.-Y.; Yamane, I.; Kim, Y.-H.; Kim, G.-S.; Suh, M.-D.; Jung, T.-S.
- Issue Date
- Feb-2005
- Keywords
- Antigen spots; Mass spectrometry; Neospora caninum; Proteome analysis; Toxoplasma gondii; Two-dimensional gel electrophoresis
- Citation
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, v.815, no.1-2, pp 305 - 314
- Pages
- 10
- Indexed
- SCOPUS
- Journal Title
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
- Volume
- 815
- Number
- 1-2
- Start Page
- 305
- End Page
- 314
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/70988
- DOI
- 10.1016/j.jchromb.2004.08.048
- ISSN
- 1570-0232
1873-376X
- Abstract
- Protein profiles of two isolates of Neospora caninum (KBA-2 and JPA1) and Toxoplasma gondii RH strain were investigated by proteomic approach. Approximately, 78% of protein spots on two-dimensional gel electrophoresis (2-DE) profiles and 80% of antigen spots on 2-DE immunoblotting profiles were exhibited to share the same pI and Mr between KBA-2 and JPA1 of N. caninum. On the other hand, a total of 30 antigen spots of T. gondii were recognized on 2-DE immunoblotting profile using rabbit antiserum against N. caninum KBA-2. A number of homologue proteins, such as heat shock protein 70, tubulin α- and β-chain, putative protein disulfide isomerase, actin, enolase and 14-3-3 protein homologue are believed as the conserved proteins in both N. caninum and T. gondii. On the contrary, NcSUB1, NcGRA2 and NCDG1 (NcGRA7) might be the species-specific proteins for N. caninum tachyzoites. The present study showed that the high degree of similarity between N. caninum isolates (KBA-2 and JPA1), whereas large differences between N. caninum and T. gondii were noticed by proteome comparisons. © 2004 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
- 의학계열 > 수의학과 > Journal Articles

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.