연교의 항산화 효과 연구Anti-oxidant effect of forsythia suspensa on cellular damage in the chronic disease
- Other Titles
- Anti-oxidant effect of forsythia suspensa on cellular damage in the chronic disease
- Authors
- 김영은; 김민진; 배수진; 박선빈; 박선동; 박광일; 김영우
- Issue Date
- Feb-2024
- Publisher
- 대한한의학방제학회
- Keywords
- Forsythiae suspensa; AMPK; YAP; oxidative stress; ROS
- Citation
- 대한한의학 방제학회지, v.32, no.1, pp 51 - 61
- Pages
- 11
- Indexed
- KCI
- Journal Title
- 대한한의학 방제학회지
- Volume
- 32
- Number
- 1
- Start Page
- 51
- End Page
- 61
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/70340
- ISSN
- 1229-1218
2288-5641
- Abstract
- Objectives : This study induced oxidative stress in HepG2 cells by treating them with AA+iron and investigated the effects of forsythia suspensa extract on this stress, as well as elucidated the molecular mechanisms underlying its hepatoprotective effects.
Methods : To confirm the antioxidative effects of FSE, HepG2 cells were induced with AA+iron to induce oxidative stress, followed by MTT assay. Additionally, the effect of FSE in reducing the increased ROS levels and mitochondrial damage induced by AA+iron in HepG2 cells was confirmed using FACS. Furthermore, western blot analysis were conducted to investigate the molecular mechanisms underlying the hepatoprotective effects of FSE.
Results : FSE increased the decreased cell viability induced by AA+iron. Additionally, FSE normalized the expression of apoptosis-related proteins induced by AA+iron. The elevated ROS levels in HepG2 cells induced by AA+iron were reduced by FSE, and the increase in Rh123-negative cells induced by AA+iron was attenuated by FSE. Moreover, FSE activated the protein expression of AMPK and its related phosphorylating enzymes, LKB1 and ACC. Furthermore, FSE activated YAP and its upstream phosphorylating enzyme, LATS1.
Conclusions : These results demonstrate that FSE has an inhibitory effect on oxidative stress induced by AA+iron and may have potential hepatoprotective effects.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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