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Identification of Autoantigens in Pediatric Gastric Juicesopen access

Authors
Youn, Hee-ShangJun, Jin-SuYeom, Jung SookPark, Ji SookLim, Jae-YoungWoo, Hyang-OkYang, Jung-WookBaik, Seung-ChulLee, Woo-KonSeo, Ji-Hyun
Issue Date
Jan-2024
Publisher
Korean Society of Pediartic Gastroenterology, Hepatology and Nutrition
Keywords
Autoantigens; Children; Gastric juice; Gastritis; Helicobacter pylori
Citation
Pediatric Gastroenterology, Hepatology and Nutrition, v.27, no.1, pp 15 - 25
Pages
11
Indexed
SCOPUS
ESCI
KCI
Journal Title
Pediatric Gastroenterology, Hepatology and Nutrition
Volume
27
Number
1
Start Page
15
End Page
25
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/69723
DOI
10.5223/pghn.2024.27.1.15
ISSN
2234-8646
2234-8840
Abstract
Purpose: This study aimed to investigate the presence of autoantigens in the gastric juices of children. Methods: Gastric juice and serum samples were obtained from 53 children <15 years of age who underwent gastric endoscopy. Among these, 8, 22, and 23 participants were in the age groups 0–5, 6–10, and 11–15 years, respectively. These samples were analyzed using two-dimensional electrophoresis (2-DE), immunoblot analysis, and matrix-assisted laser desorption ionization-time of-flight mass spectrometry. Furthermore, we reviewed the histopathological findings and urease test results and compared them with the results of 2-DE and immunoblot analysis. Results: There were no statistically significant differences in urease test positivity, grades of chronic gastritis, active gastritis, or Helicobacter pylori infiltration of the antrum and body among the three age groups. Three distinct patterns of gastric juice were observed on 2-DE. Pattern I was the most common, and pattern III was not observed below the age of 5 years. Histopathological findings were significantly different among active gastritis (p=0.037) and H. pylori infiltration (p=0.060) in the gastric body. The immunoblots showed large spots at an approximate pH of 3–4 and molecular weights of 31–45 kDa. These distinct, large positive spots were identified as gastric lipase and pepsin A and C. Conclusion: Three enzymes, which are normally secreted under acidic conditions were identified as autoantigens. Further investigation of the pathophysiology and function of autoantigens in the stomach is required. © 2024 by The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
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