BMP-2-immobilized PCL 3D printing scaffold with a leaf-stacked structure as a physically and biologically activated bone graft
- Authors
- Kim, Min Ji; Park, Jin-Ho; Seok, Ji Min; Jung, Jiwoon; Hwang, Tae Sung; Lee, Hee-Chun; Lee, Jin Ho; Park, Su A.; Byun, June Ho; Oh, Se Heang
- Issue Date
- Apr-2024
- Publisher
- IOP Publishing Ltd
- Keywords
- 3D printing; bone morphogenetic protein-2 (BMP-2); bone reconstruction; leaf-stacked structure; tissue engineering
- Citation
- Biofabrication, v.16, no.2
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biofabrication
- Volume
- 16
- Number
- 2
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/69718
- DOI
- 10.1088/1758-5090/ad2537
- ISSN
- 1758-5082
1758-5090
- Abstract
- Although three-dimensional (3D) printing techniques are used to mimic macro- and micro-structures as well as multi-structural human tissues in tissue engineering, efficient target tissue regeneration requires bioactive 3D printing scaffolds. In this study, we developed a bone morphogenetic protein-2 (BMP-2)-immobilized polycaprolactone (PCL) 3D printing scaffold with leaf-stacked structure (LSS) (3D-PLSS-BMP) as a bioactive patient-tailored bone graft. The unique LSS was introduced on the strand surface of the scaffold via heating/cooling in tetraglycol without significant deterioration in physical properties. The BMP-2 adsorbed on3D-PLSS-BMPwas continuously released from LSS over a period of 32 d. The LSS can be a microtopographical cue for improved focal cell adhesion, proliferation, and osteogenic differentiation.In vitrocell culture andin vivoanimal studies demonstrated the biological (bioactive BMP-2) and physical (microrough structure) mechanisms of3D-PLSS-BMPfor accelerated bone regeneration. Thus, bioactive molecule-immobilized 3D printing scaffold with LSS represents a promising physically and biologically activated bone graft as well as an advanced tool for widespread application in clinical and research fields. © 2024 IOP Publishing Ltd.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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