Expression of TASK-1 channel in mouse Leydig cellsopen accessExpression of TASK-1 channel in mouse Leydig cells
- Other Titles
- Expression of TASK-1 channel in mouse Leydig cells
- Authors
- 우민석; 김은진; Anjas Happy Prayoga; 김양미; 강다원
- Issue Date
- Dec-2023
- Publisher
- 사단법인 한국동물생명공학회
- Keywords
- cell death; Leydig cells; mice; TASK-1; testosterone
- Citation
- 한국동물생명공학회지, v.38, no.4, pp 291 - 299
- Pages
- 9
- Indexed
- KCI
- Journal Title
- 한국동물생명공학회지
- Volume
- 38
- Number
- 4
- Start Page
- 291
- End Page
- 299
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/69383
- DOI
- 10.12750/JARB.38.4.291
- ISSN
- 2671-4639
- Abstract
- Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis.
Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker’s effect on testosterone secretion was evaluated by measuring testosterone levels.
Results: Immunohistochemical analysis revealed a predominant presence of TASK- 1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LHinduced testosterone secretion.
Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medicine > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/69383)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.