Enhancing polyethylene degradation: a novel bioprocess approach using Acinetobacter nosocomialis pseudo-resting cells
- Authors
- Seong, Hyeon Jeong; Kim, Hyejin; Ko, Young-Joon; Yao, Zhuang; Baek, Song-Bum; Kim, Nam-Jung; Jang, Yu-Sin
- Issue Date
- Dec-2024
- Publisher
- Springer Science and Business Media Deutschland GmbH
- Keywords
- Acinetobacter; Biodegradation; GNU001; Polyethylene; Pseudo-resting cells
- Citation
- Applied Microbiology and Biotechnology, v.108, no.1, pp 1 - 12
- Pages
- 12
- Indexed
- SCOPUS
- Journal Title
- Applied Microbiology and Biotechnology
- Volume
- 108
- Number
- 1
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/69354
- DOI
- 10.1007/s00253-023-12930-5
- ISSN
- 0175-7598
1432-0614
- Abstract
- Abstract: Despite the discovery of several bacteria capable of interacting with polymers, the activity of the natural bacterial isolates is limited. Furthermore, there is a lack of knowledge regarding the development of bioprocesses for polyethylene (PE) degradation. Here, we report a bioprocess using pseudo-resting cells for efficient degradation of PE. The bacterial strain Acinetobacter nosocomialis was isolated from PE-containing landfills and characterized using low-density PE (LDPE) surface oxidation when incubated with LDPE. We optimized culture conditions to generate catalytic pseudo-resting cells of A. nosocomialis that are capable of degrading LDPE films in a bioreactor. After 28 days of bioreactor operation using pseudo-resting cells of A. nosocomialis, we observed the formation of holes on the PE film (39 holes per 217 cm2, a maximum diameter of 1440 μm). This study highlights the potential of bacteria as biocatalysts for the development of PE degradation processes. Key points: • New bioprocess has been proposed to degrade polyethylene (PE). • Process with pseudo-resting cells results in the formation of holes in PE film. • We demonstrated PE degradation using A. nosocomialis as a biocatalyst. © 2024, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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