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PDGFRβ Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear Localizationopen access

Authors
Perera, Chalani DilshaniIdrees, MuhammadKhan, Abdul MajidHaider, ZaheerUllah, SafeerKang, Ji-SuLee, Seo-HyunKang, Seon-MinKong, Il-Keun
Issue Date
Dec-2023
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Keywords
activator; bovine blastocysts; embryonic genome activation; NFYA; PDGFRβ
Citation
International Journal of Molecular Sciences, v.24, no.23
Indexed
SCIE
SCOPUS
Journal Title
International Journal of Molecular Sciences
Volume
24
Number
23
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/68945
DOI
10.3390/ijms242317047
ISSN
1661-6596
1422-0067
Abstract
Embryonic genome activation (EGA) is a critical step during embryonic development. Several transcription factors have been identified that play major roles in initiating EGA; however, this gradual and complex mechanism still needs to be explored. In this study, we investigated the role of nuclear transcription factor Y subunit A (NFYA) in bovine EGA and bovine embryonic development and its relationship with the platelet-derived growth factor receptor-β (PDGFRβ) by using a potent selective activator (PDGF-BB) and inhibitor (CP-673451) of PDGF receptors. Activation and inhibition of PDGFRβ using PDGF-BB and CP-673451 revealed that NFYA expression is significantly (p < 0.05) affected by the PDGFRβ. In addition, PDGFRβ mRNA expression was significantly increased (p < 0.05) in the activator group and significantly decreased (p < 0.05) in the inhibitor group when compared with PDGFRα. Downregulation of NFYA following PDGFRβ inhibition was associated with the expression of critical EGA-related genes, bovine embryo development rate, and implantation potential. Moreover, ROS and mitochondrial apoptosis levels and expression of pluripotency-related markers necessary for inner cell mass development were also significantly (p < 0.05) affected by the downregulation of NFYA while interrupting trophoblast cell (CDX2) differentiation. In conclusion, the PDGFRβ-NFYA axis is critical for bovine embryonic genome activation and embryonic development. © 2023 by the authors.
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