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Molecular genetic characterisation and expression profiling of calpain 3 transcripts in red sea bream (Pagrus major)

Authors
Hwang, Seong DonChoi, Kwang-MinHwang, Jee YounKwon, Mun-GyeongJeong, Ji-MinSeo, Jung SooJee, Bo-YeongPark, Chan-Il
Issue Date
Mar-2020
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Keywords
Pagrus major; Streptococcus iniae; Edwardsiella piscicida; Red sea bream iridovirus; Calpain 3
Citation
FISH & SHELLFISH IMMUNOLOGY, v.98, pp.19 - 24
Indexed
SCIE
SCOPUS
Journal Title
FISH & SHELLFISH IMMUNOLOGY
Volume
98
Start Page
19
End Page
24
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6878
DOI
10.1016/j.fsi.2019.12.090
ISSN
1050-4648
Abstract
Calpains (CAPNs) belong to the papain superfamily of cysteine proteases, and they are calcium-dependent cytoplasmic cysteine proteases that regulate a variety of physiological processes. We obtained the sequence of CAPN3 from an NGS-based analysis of Pagrus major (PmCAPN3) and confirmed the conserved molecular biological properties in the predicted amino acid sequence. The amino acid sequence and predicted domains of CAPN3 were found to be highly conserved in all of the examined species, and one catalytic domain and four calcium binding sites were identified. In healthy P. major, the PmCAPN3 mRNA was most abundantly expressed in the muscle and skin, and ubiquitously expressed in the other tissues used in the experiment. After artificial infections with fish pathogens, significant changes in its expression levels were found in immune-related tissues, most of showed upregulation. In particular, the highest level of expression was found in the liver, a tissue associated with protease activity. Taken together, these results suggest a physiological activity for PmCAPN3 in P. major and reveal functional possibilities that have not yet been reported in the immune system.
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해양과학대학 (해양생명과학과)
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