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Subpopulations of miniature pig mesenchymal stromal cells with different differentiation potentials differ in the expression of octamer-binding transcription factor 4 and sex determining region Y-box 2open access

Authors
Jeon, RyounghoonPark, SungjoLee, Sung-LimRho, Gyu-Jin
Issue Date
Mar-2020
Publisher
아세아·태평양축산학회
Keywords
Miniature Pig; Mesenchymal Stromal Cell (MSC) Subpopulations; Octamer-binding Transcription Factor 4 (OCT4); Sex Determining Region Y-box 2 (SOX2); Aging
Citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.33, no.3, pp 515 - 524
Pages
10
Indexed
SCIE
SCOPUS
KCI
Journal Title
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
Volume
33
Number
3
Start Page
515
End Page
524
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/6877
DOI
10.5713/ajas.19.0416
ISSN
1011-2367
1976-5517
Abstract
Objective: Human mesenchymal stromal cells (MSCs) exhibit variable differentiation potential and can be divided accordingly into distinct subpopulations whose ratios vary with donor age. However, it is unknown whether the same is true in pigs. This study investigated MSC subpopulations in miniature pig and compared their characteristics in young (2 to 3 months) and adult (27 to 35 months) pigs. Methods: Osteogenic, chondrogenic, and adipogenic capacity of isolated MSCs was evaluated by von Kossa, Alcian blue, and oil red O staining, respectively. Cell surface antigen expression was determined by flow cytometry. Proliferative capacity was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Expression of marker genes was detected by quantitative real-time polymerase chain reaction. Results: Porcine MSCs comprised cells with trilineage and bilineage differentiation potential (tMSCs and bMSCs, respectively) and non-differentiating stromal cells (NDSCs). The tMSC and bMSC fractions were smaller in adult than in young pigs (63.0% vs 71.2% and 11.6% vs 24.0%, respectively, p<0.05); NDSCs showed the opposite trend (25.4% vs 4.8%; p<0.05). Subpopulations showed no differences in morphology, cell surface antigen expression, or proliferative capacity, but octamer-binding transcription factor 4 (OCT4) expression was higher in tMSCs than in bMSCs and NDSCs (p<0.05), whereas sex determining region Y-box 2 (SOX2) expression was higher in tMSCs and bMSCs than in NDSCs (p<0.05). Aging had no effect on these trends. Conclusion: Porcine MSCs comprise distinct subpopulations that differ in their differentiation potential and OCT4 and SOX2 expression. Aging does not affect the characteristics of each subpopulation but alters their ratios.
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