Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infectionopen access

Authors
Agidigbi, Taiwo SamuelKwon, Hyuk-KwonKnight, James R. R.Zhao, DejianLee, Francis Y. Y.Oh, Irvin
Issue Date
Jun-2023
Publisher
FRONTIERS MEDIA SA
Keywords
DFU; S; aureus-infection; transcriptome; PBMC; wound healing; host-immunity
Citation
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v.13
Indexed
SCIE
SCOPUS
Journal Title
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume
13
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/68723
DOI
10.3389/fcimb.2023.1198115
ISSN
2235-2988
Abstract
IntroductionInfection in diabetic foot ulcers (DFUs) is one of the major complications associated with patients with diabetes. Staphylococcus aureus is the most common offending pathogen in patients with infected DFU. Previous studies have suggested the application of species-specific antibodies against S. aureus for diagnosis and monitoring treatment response. Early and accurate identification of the main pathogen is critical for management of DFU infection. Understanding the host immune response against species-specific infection may facilitate diagnosis and may suggest potential intervention options to promote healing infected DFUs. We sought to investigate evolving host transcriptome associated with surgical treatment of S. aureus- infected DFU. MethodsThis study compared the transcriptome profile of 21 patients with S. aureus- infected DFU who underwent initial foot salvage therapy with irrigation and debridement followed by intravenous antibiotic therapy. Blood samples were collected at the recruitment (0 weeks) and 8 weeks after therapy to isolate peripheral blood mononuclear cells (PBMCs). We analyzed the PBMC expression of transcriptomes at two different time points (0 versus 8 weeks). Subjects were further divided into two groups at 8 weeks: healed (n = 17, 80.95%) versus non-healed (n = 4, 19.05%) based on the wound healing status. DESeq2 differential gene analysis was performed. Results and discussionAn increased expression of IGHG1, IGHG2, IGHG3, IGLV3-21, and IGLV6-57 was noted during active infection at 0 weeks compared with that at 8 weeks. Lysine- and arginine-rich histones (HIST1H2AJ, HIST1H2AL, HIST1H2BM, HIST1H3B, and HIST1H3G) were upregulated at the initial phase of active infection at 0 weeks. CD177 and RRM2 were also upregulated at the initial phase of active infection (0 weeks) compared with that at 8 weeks of follow-up. Genes of heat shock protein members (HSPA1A, HSPE1, and HSP90B1) were high in not healed patients compared with that in healed patients 8 weeks after therapy. The outcome of our study suggests that the identification of genes evolution based on a transcriptomic profiling could be a useful tool for diagnosing infection and assessing severity and host immune response to therapies.
Files in This Item
There are no files associated with this item.
Appears in
Collections
자연과학대학 > Division of Life Sciences > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE