Lipofundin MCT/LCT Inhibits Levcromakalim-Induced Vasodilation by Inhibiting Endothelial Nitric Oxide Releaseopen access
- Lee, Soo Hee; Kang, Dawon; Ok, Seong-Ho; Kim, Ji-Yoon; Bae, Sung Il; Hwang, Yeran; Park, Kyeong-Eon; Kim, Jong Won; Sohn, Ju-Tae
- Issue Date
- lipid emulsion; levcromakalim; nitric oxide; ATP-sensitive potassium channel
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.5
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- The goal of this study was to examine the effect of lipid emulsion on the vasodilation induced by ATP-sensitive potassium (K-ATP) channels in isolated rat aortae and the underlying mechanism. The effects of Intralipid, containing 100% long-chain fatty acids, and Lipofundin MCT/LCT, containing 50% long-chain fatty acids plus 50% medium-chain fatty acids, on the vasodilation induced by levcromakalim in endothelium-intact aorta with or without N-W-nitro-L-arginine methyl ester (L-NAME) and in endothelium-denuded aorta were examined. The effects of L-arginine, L-NAME, glibenclamide, and Lipofundin MCT/LCT, alone or combined, on the levcromakalim-induced vasodilation were examined. Lipofundin MCT/LCT inhibited the levcromakalim-induced vasodilation of isolated endothelium-intact aortae, whereas Intralipid did not. In addition, Lipofundin MCT/LCT had no effect on the levcromakalim-induced vasodilation of endothelium-denuded rat aortae and endothelium-intact aortae with L-NAME. L-arginine and Lipofundin MCT/LCT produced more levcromakalim-induced vasodilation than Lipofundin MCT/LCT alone. Glibenclamide inhibited levcromakalim-induced vasodilation. Levcromakalim did not significantly alter endothelial nitric oxide synthase phosphorylation, whereas Lipofundin MCT/LCT decreased cyclic guanosine monophosphate. Lipofundin MCT/LCT did not significantly alter levcromakalim-induced membrane hyperpolarization. Taken together, these results suggest that Lipofundin MCT/LCT inhibits the vasodilation induced by levcromakalim by inhibiting basally released endothelial nitric oxide, which seems to occur through medium-chain fatty acids.
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- College of Medicine > Department of Medicine > Journal Articles
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