Detailed Information

Cited 23 time in webofscience Cited 33 time in scopus
Metadata Downloads

The Importance of Porins and beta-Lactamase in Outer Membrane Vesicles on the Hydrolysis of beta-Lactam Antibioticsopen access

Authors
Kim, Si WonLee, Jung SeokPark, Seong BinLee, Ae RinJung, Jae WookChun, Jin HongLazarte, Jassy Mary S.Kim, JaesungSeo, Jong-SuKim, Jong-HwanSong, Jong-WookHa, Min WooThompson, Kim D.Lee, Chang-RoJung, MyunghwanJung, Tae Sung
Issue Date
Apr-2020
Publisher
MDPI
Keywords
outer membrane vesicles (OMVs); beta-lactamase; porin; beta-lactam antibiotic; Escherichia coli; hydrolysis
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.8
Indexed
SCIE
SCOPUS
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume
21
Number
8
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6793
DOI
10.3390/ijms21082822
ISSN
1661-6596
Abstract
Gram-negative bacteria have an outer membrane inhibiting the entry of antibiotics. Porins, found within the outer membrane, are involved in regulating the permeability of beta-lactam antibiotics. beta-lactamases are enzymes that are able to inactivate the antibacterial properties of beta-lactam antibiotics. Interestingly, porins and beta-lactamase are found in outer membrane vesicles (OMVs) of beta-lactam-resistant Escherichia coli and may be involved in the survival of susceptible strains of E. coli in the presence of antibiotics, through the hydrolysis of the beta-lactam antibiotic. In this study, OMVs isolated from beta-lactam-resistant E. coli and from mutants, lacking porin or beta-lactamase, were evaluated to establish if the porins or beta-lactamase in OMVs were involved in the degradation of beta-lactam antibiotics. OMVs isolated from E. coli deficient in beta-lactamase did not show any degradation ability against beta-lactam antibiotics, while OMVs lacking OmpC or OmpF showed significantly lower levels of hydrolyzing activity than OMVs from parent E. coli. These data reveal an important role of OMVs in bacterial defense mechanisms demonstrating that the OmpC and OmpF proteins allow permeation of beta-lactam antibiotics into the lumen of OMVs, and antibiotics that enter the OMVs can be degraded by beta-lactamase.
Files in This Item
There are no files associated with this item.
Appears in
Collections
수의과대학 > Department of Veterinary Medicine > Journal Articles
College of Medicine > Department of Medicine > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jung, Myung Hwan photo

Jung, Myung Hwan
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE