Pretreatment of Anthocyanin from the Fruit of Vitis coignetiae Pulliat Acts as a Potent Inhibitor of TNF-alpha Effect by Inhibiting NF-kappa B-Regulated Genes in Human Breast Cancer Cellsopen access
- Paramanantham, Anjugam; Kim, Min Jeong; Jung, Eun Joo; Nagappan, Arulkumar; Yun, Jeong Won; Kim, Hye Jung; Shin, Sung Chul; Kim, Gon Sup; Lee, Won Sup
- Issue Date
- anthocyanins; Vitis coignetiae Pulliat; NF-kappa B; breast cancer
- MOLECULES, v.25, no.10
- Journal Title
- Vitis coignetiaePulliat (Meoru in Korea) has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. Evidence suggests that NF-kappa B activation is mainly involved in cancer cell proliferation, invasion, angiogenesis, and metastasis. TNF-alpha also enhances the inflammatory process in tumor development. Recently, flavonoids from plants have been reported to have inhibitory effects on NF-kappa B activities. We investigated the effects of anthocyanins extracted from the fruits of Vitis coignetiae Pulliat (AIM, anthocyanins isolated from Meoru (AIM)) on TNF-alpha -induced NF-kappa B activities in MCF-7 human breast cancer cells and the molecules involved in AIM-induced anti-cancer effects, especially on cancer metastasis. We performed cell viability assay, gelatin zymography, invasion assay, and western blot analysis to unravel the anti-NF-kappa B activity of AIMs on MCF-7 cells. AIM suppressed the TNF-alpha effects on the NF-kappa B-regulated proteins involved in cancer cell proliferation (COX-2, C-myc), invasion, and angiogenesis (MMP-2, MMP9, ICAM-1, and VEGF). AIM also increased the expression of E-cadherin, which is one of the hallmarks of the epithelial-mesenchymal transition (EMT) process. In conclusion, this study demonstrates that the anthocyanins isolated from the fruits of Vitis coignetiae Pulliat acts as an inhibitor of TNF-alpha induced NF-kappa B activation, and subsequent downstream molecules involved in cancer proliferation, invasion, adhesion, angiogenesis, and thus have anti-metastatic activities in MCF-7 breast cancer cells.
- Files in This Item
- There are no files associated with this item.
- Appears in
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
- College of Medicine > Department of Medicine > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.