Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Miceopen access
- Reyes, Alisha Wehdnesday Bernardo; Vu, Son Hai; Tran Xuan Ngoc Huy; Min, Wongi; Lee, Hu Jang; Chang, Hong Hee; Lee, John Hwa; Kim, Suk
- Issue Date
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Brucella abortus; cytokines; invasion; linoleic acid; spleen weight
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.30, no.5, pp.642 - 648
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Start Page
- End Page
- In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocyte RAW264.7 cells, particularly during the pathogen's invasion and intracellular growth in these cells, as well as in murine model BALB/c mice focusing on bacterial splenic proliferation and immunoregulatory activities. LA inhibited the growth of Brucella in a dose- and time-dependent manner. The ability of the pathogen to enter the phagocytes was inhibited as was its survival within these cells. This was accompanied by increased nitrite accumulation in these cells at 24 h post-infection. The concentration of LA used in the present study did not affect the total body weight or liver function of the mice. During Brucella infection, the total splenic weight of these animals was not changed; rather, resistance to bacterial proliferation was enhanced in the spleen. Furthermore, mice treated with LA displayed elevated levels of IL-12 and IFN-gamma but reduced levels of IL-10 during infection. The findings in this study showed the regulatory role of LA against B. abortus infection suggesting its potential use in designing intervention strategy for brucellosis.
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- 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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