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Cited 7 time in webofscience Cited 8 time in scopus
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Molecular cloning and functional analysis of deubiquitinase CYLD in rainbow trout, Oncorhynchus mykiss

Authors
Jang, Ju HyeLee, Hyang MiKim, HyunCho, Ju Hyun
Issue Date
Jun-2020
Publisher
Academic Press
Keywords
CYLD; Deubiquitinase; Innate immunity; Teleost; Rainbow trout; RTH-149
Citation
Fish and Shellfish Immunology, v.101, pp 135 - 142
Pages
8
Indexed
SCIE
SCOPUS
Journal Title
Fish and Shellfish Immunology
Volume
101
Start Page
135
End Page
142
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/6568
DOI
10.1016/j.fsi.2020.03.058
ISSN
1050-4648
1095-9947
Abstract
Deubiquitinase cylindromatosis (CYLD) inhibits MAPK and NF-KB activation pathways by deubiquitinating upstream regulatory factors. Although CYLD has been identified and actively studied in mammals, nothing is known about its putative function in fish. In this study, we identified the gene encoding CYLD (OmCYLD) from rainbow trout, Oncorhynchus mykiss, and examined its role during pathogenic infections. The deduced amino acid sequence of OmCYLD contains conserved CAP-Gly and USP domains. In RTH-149 cells, the expression of OmCYLD was increased by stimulation with Edwardsiella tarda and Streptococcus iniae. Gain-of-function and lossof-function experiments showed that OmCYLD down-regulates the activation of MAPK and NF-kappa B and the expression of pro-inflammatory cytokines in E. tarda-stimulated RTH-149 cells. These findings suggest that OmCYLD might function like those of mammals to negatively regulate bacteria-triggered signaling pathway in fish.
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Cho, Ju Hyun
대학원 (응용생명과학부)
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