Parthenolide Has Negative Effects on In Vitro Enhanced Osteogenic Phenotypes by Inflammatory Cytokine TNF-alpha via Inhibiting JNK Signalingopen access
- Authors
- Park, Jin-Ho; Kang, Young-Hoon; Hwang, Sun-Chul; Oh, Se Heang; Byun, June-Ho
- Issue Date
- Aug-2020
- Publisher
- MDPI
- Keywords
- parthenolide; TNF-alpha; periosteum-derived cells; osteoblastic differentiation; JNK signaling
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.15, pp 1 - 14
- Pages
- 14
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 21
- Number
- 15
- Start Page
- 1
- End Page
- 14
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/6332
- DOI
- 10.3390/ijms21155433
- ISSN
- 1661-6596
1422-0067
- Abstract
- Nuclear factor kappa B (NF-kappa B) regulates inflammatory gene expression and represents a likely target for novel disease treatment approaches, including skeletal disorders. Several plant-derived sesquiterpene lactones can inhibit the activation of NF-kappa B. Parthenolide (PTL) is an abundant sesquiterpene lactone, found in Mexican Indian Asteraceae family plants, with reported anti-inflammatory activity, through the inhibition of a common step in the NF-kappa B activation pathway. This study examined the effects of PTL on the enhanced, in vitro, osteogenic phenotypes of human periosteum-derived cells (hPDCs), mediated by the inflammatory cytokine tumor necrosis factor (TNF)-alpha. PTL had no significant effects on hPDC viability or osteoblastic activities, whereas TNF-alpha had positive effects on the in vitro osteoblastic differentiation of hPDCs. c-Jun N-terminal kinase (JNK) signaling played an important role in the enhanced osteoblastic differentiation of TNF-alpha-treated hPDCs. Treatment with 1 mu M PTL did not affect TNF-alpha-treated hPDCs; however, 5 and 10 mu M PTL treatment decreased the histochemical detection and activity of alkaline phosphatase (ALP), alizarin red-positive mineralization, and the expression of ALP and osteocalcin mRNA. JNK phosphorylation decreased significantly in TNF-alpha-treated hPDCs pretreated with PTL. These results suggested that PTL exerts negative effects on the increased osteoblastic differentiation of TNF-alpha-treated hPDCs by inhibiting JNK signaling.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medicine > Journal Articles

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.