Overfeeding-Induced Obesity Could Cause Potential Immuno-Physiological Disorders in Rainbow Trout (Oncorhynchus mykiss)

Abstract

Simple Summary In this study, we have successfully generated overfeeding-induced obesity in rainbow trout and demonstrated that the overfeeding regime causes adverse effects on the health status of fish. What we found in this study is that fish in the overfed group harbor enlarged liver and macrophages due to high lipid accumulation. The expression of IL-10, CD36, TLR2, and HSP70 (stress and/or obesity-related genes) was significantly upregulated in overfed fish. Moreover, oxidized low-density lipoprotein (OxLDL), which is known to be produced more in obese individuals, caused apoptosis of trout lymphocyte. These results clearly indicate that overfeeding-induced obesity can be the source of stress and cause immuno-physiological disorders in rainbow trout. Awareness and knowledge of farmers on the relationship between overfeeding and fish health might contribute to increased disease resistance and aquaculture production. Although over-nutrition from overfeeding-induced obesity is known to be highly associated with metabolic and immunological disorders in humans, little is known about overfeeding-induced obesity in fish farming. The purpose of this study was to investigate changes in immuno-physiological parameters, to better understand the potential risk of overfeeding-induced obesity in fish. Commercial feed was provided to fish in the overfed group until they refuse to eat, but fish in the control group was fed with the feed at 1% bodyweight per day. The hemato-serological, histological, and immunological changes were observed at weeks 2 and 8. Rainbow trout leukocytes were co-incubated with oxidized low-density lipoprotein (OxLDL), and the phagocytes engulfing the OxLDL and the presence of apoptotic cells were evaluated. The body weight, body mass index (BMI), and hepatosomatic index (HSI) index were significantly higher in the overfed group, and high lipid accumulation and fatty changes were also observed in their livers, indicating that the feeding regime used in this study led to overfeeding-induced obesity. Likewise, much higher numbers of and larger vacuoles were observed in overfed fish macrophages, showing unclear boundaries between the cytoplasm and extracellular space. In the overfed group, the expression of IL-10, HSP70, TLR2, and CD36 was significantly higher, and lymphocyte apoptosis was more evident, indicating that overfeeding-induced obese fish might have immunologic disorders. This was the first study to demonstrate that overfeeding-induced obesity could cause an immune-physiological imbalance in rainbow trout, making them more vulnerable to infectious diseases and various stressful conditions. This study will contribute to improvements in fish nutrition, feeding practices, fish nutrition, and disease prevention in the aquaculture industry.