Antioxidant and Anti-Inflammatory Effects of NCW Peptide from Clam Worm (Marphysa sanguinea)open access
- Park, Young Ran; Park, Chan-Il; Soh, Yunjo
- Issue Date
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Novel peptide; clam worm; antioxidant; anti-inflammation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.30, no.9, pp.1387 - 1394
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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- End Page
- Clam worms (Marphysa sanguinea) are a rich source of bioactive components such as the antibacterial peptide, perinerin. In the present study, we explored the physiological activities of a novel NCWPFQGVPLGFQAPP peptide (NCW peptide), which was purified from clam worm extract through high-performance liquid chromatography. Tandem mass spectrometry (MS/MS) revealed that NCW was a new peptide with a molecular weight of 1757.86 kDa. Moreover, NCW peptide exhibited significant antioxidant effects, causing a 50% inhibition of DPPH radical at a concentration of 20 mu M without showing any cytotoxicity. These were associated with a reduction in the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in LPS-stimulated RAW264.7 cells. Furthermore, NCW peptide exhibited anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via inhibition of the abnormal production of pro-inflammatory cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). These anti-inflammatory effects of NCW peptide were associated with the inhibition of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). Our results therefore suggest that this novel NCW peptide with antioxidant and anti-inflammatory effects could be a good therapeutic agent against inflammation-related diseases.
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- 해양과학대학 > 양식생명과학과 > Journal Articles
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