ERH overexpression is associated with decreased cell migration and invasion and a good prognosis in gastric canceropen access
- Park, Ji-Ho; Park, Miyeong; Park, Sun Yi; Lee, Young-Joon; Hong, Soon-Chan; Jung, Eun-Jung; Ju, Young-Tae; Jeong, Chi-Young; Kim, Ju-Yeon; Ko, Gyung Hyuck; Hah, Young-Sool; Jeong, Sang-Ho
- Issue Date
- AME PUBL CO
- Stomach neoplasm; enhancer of rudimentary homolog (ERH); immunohistochemistry (IHC); biomarker
- TRANSLATIONAL CANCER RESEARCH, v.9, no.9, pp.5281 - 5291
- Journal Title
- TRANSLATIONAL CANCER RESEARCH
- Start Page
- End Page
- Background: The enhancer of rudimentary homolog (ERH) protein is implicated in transcriptional regulation, cell cycle progression, and malignancy. We previously conducted a proteomics analysis using gastric cancer (GC) tissues and identified ERH as a biomarker candidate. The aim of this study was to investigate whether ERH may be useful as a prognostic marker for GC. Methods: Surgically resected GC tissue specimens were obtained from 327 patients who underwent gastrectomy at Gyeongsang National University Hospital. Immunohistochemistry (IHC) was used to validate ERH as a prognostic marker in these tissues. SNU601 and MKN74 cells with siRNA-mediated knockdown of ERH expression and ERH-overexpressing SNU601 and MKN74 knock-in cells were used for analysis of ERH function. Results: ERH was overexpressed in stomach cancer tissues compared with normal tissues according to proteomics analysis (n=29, P<0.01) of patient samples. Based on IHC, patients with tumors overexpressing ERH had lower T stage and lower TNM stage classifications, lower cancer recurrence rates and longer survival times than did patients with tumors showing low expression of ERH (P=0.04). In vitro, forced expression of ERH significantly decreased GC cell migration and invasion, and depletion of ERH triggered GC cell migration and invasion but had no effect on proliferation in vitro. Conclusions: The findings from the present study show that ERH is associated with decreased cancer cell migration and invasion, suggesting that overexpression of ERH may serve as a marker of good prognosis for patients with GC.
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- College of Medicine > Department of Medicine > Journal Articles
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