Clonal Distribution of Clindamycin-Resistant Erythromycin-Susceptible (CRES) Streptococcus agalactiae in Korea Based on Whole Genome Sequencesopen access
- Authors
- Takahashi, Takashi; Maeda, Takahiro; Lee, Seungjun; Lee, Dong-Hyun; Kim, Sunjoo
- Issue Date
- Sep-2020
- Publisher
- KOREAN SOC LABORATORY MEDICINE
- Keywords
- Streptococcus agalactiae; Group B streptococci; Antimicrobial resistance; Whole genome sequencing; Sequence types; Clonal distribution; CRES (clindamycin-resistant erythromycin-susceptible)
- Citation
- ANNALS OF LABORATORY MEDICINE, v.40, no.5, pp.370 - 381
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- ANNALS OF LABORATORY MEDICINE
- Volume
- 40
- Number
- 5
- Start Page
- 370
- End Page
- 381
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/6215
- DOI
- 10.3343/alm.2020.40.5.370
- ISSN
- 2234-3806
- Abstract
- Background: The clindamycin-resistant erythromycin-susceptible (CRES) phenotype is rare in Streptococcus agalactiae (group B streptococci). We aimed to determine the molecular characteristics of CRES S. agalactiae using whole genome sequencing (WGS). Methods: Sixty-six S. agalactiae isolates obtained from blood (N=26), cerebrospinal fluid (N=10), urine (N=17), and vaginal discharge (N=13) between 2010 and 2017 in Korea were subjected to WGS. Based on the WGS data, we analyzed antimicrobial resistance (AMR) determinants, sequence types (STs), capsular polysaccharide (CPS) genotypes, and virulence gene profiles, and constructed a phylogenetic tree. We included the clinda-mycin-susceptible erythromycin-resistant (CSER) phenotype for comparison. Results: We identified seven CRES S. agalactiae isolates from urine (N=5) and vaginal discharge (N=2) collected between 2010 and 2011. All CRES isolates harbored AMR de-terminants of Inu(B), Isa(E), and aac(6')-aph(2 ''), revealed ST19 and CPS genotype III, and had a virulence gene profile of rib-Imb-cylE. Phylogenetic tree analysis revealed that all CRES isolates belonged to the same cluster, suggesting a clonal distribution. In con-trast, seven CSER isolates showed a diverse distribution and clustered separately from the CRES isolates. Conclusions: CRES isolates collected between 2010 and 2011 showed a unique cluster with ST19 and CPS genotype III in Korea. This is the first report on WGS-based characteristics of S. agalactiae in Korea.
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