산수유의 Prebiotics 특성 및 Lipopolysaccharide로 유도된 장 상피세포 보호 효과

Abstract

To evaluate the prebiotic effect of water extract from Corni fructus (WCF), we measured the growth curves, titratable acidity and short-chain fatty acid (SCFAs) contents in Lactobacillus reuteri (L. reuteri) and Lactobacillus brevis strains. After 24 h incubation, growth curve of the WCF-treated L. reuteri strain showed a relatively higher viable count and titratable acidity, as compared to the fructo-oligosaccharide (FOS)-treated strain (positive control). Considering that SCFA contents are useful metabolites for gut health, our results revealed higher contents of acetate in the WCF-treated strain (1,235.98±62.71 mM) than the FOS-treated strain (763.57±5.38 mM) of L. reuteri. DCF-DA [intracellular reactive oxygen species (ROS) content] and MTT assays [cell viability on lipopolysaccharide (LPS)-induced cytotoxicity] were applied in human colon epithelial HT-29 cells to determine the cell protective effect of the WCF. The results indicate that WCF effectively decreases the intracellular ROS contents and increases cell viability in LPS-induced HT-29 cells. To confirm the inhibitory effect of WCF on LPS-induced inflammation, we examined the expression levels of toll-like receptor 4 (TLR4) and nuclear factor-ĸB (NF-κB). Regulation of the LPS-induced inflammatory reaction by WCF was achieved by decreasing the expressions of TLR4 and NF-ĸB in HT-29 cells. Exposure to WCF also inhibited the cell permeability injury, as determined by increased expressions of tight junction proteins (occludin and claudin-1) in LPS-induced HT-29 cells, indicating the anti-inflammatory effect of WCF. Finally, morroniside and loganin, the major compound iridoid glycosides in the WCF, were analyzed by high-performance liquid chromatography. Taken together, our results indicate that Corni fructus is a potentially good natural source as a prebiotic to prevent intestinal dysfunction. ? 2021 Korean Society of Food Science and Nutrition. All rights reserved.