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Biosynthesis of trifolin, a bioactive flavonoid by biotransformation

Authors
Noh, H.-R.Kang, J.-Y.Kim, B.-G.
Issue Date
2021
Publisher
Korean Society for Applied Biological Chemistry
Keywords
Biotransformation; Co-culture; Flavonoids; Flavonol synthase; Glycosyltransferase; Trifolin; UDP-galactose 4-epimerase
Citation
Journal of Applied Biological Chemistry, v.64, no.3, pp 309 - 316
Pages
8
Indexed
SCOPUS
KCI
Journal Title
Journal of Applied Biological Chemistry
Volume
64
Number
3
Start Page
309
End Page
316
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/5540
DOI
10.3839/jabc.2021.042
ISSN
1976-0442
2234-7941
Abstract
Kaempferol 3-O-galactoside (Trifolin), a member of the flavonol group, has been reported to have anticancer effects against promyelocytic leukemia, histocytic lymphoma, skin melanoma and lung cancer. Trifolin has been extracted and used from several plants, but the extraction process is complicated and the final yield is low. Biotransformation is an alternative tool to produce high value-added chemicals from inexpensive compounds. To synthesis trifolin from naringenin, three genes (PeFLS and OsUGE-PhUGT) were introduced into Escherichia coli, respectively. In order to synthesis trifolin from naringenin, a co-culture fermentation system was established by optimizing the cell concentration, biotransformation temperature and medium, isopropyl-β-D-thiogalactoside (IPTG) concentration, substrate supply concentration, and recombinant protein induction time. The established optimal conditions for trifolin production were a 3:1 ratio of BL-UGTE to BL-FLS, induction of recombinant protein at 25oC for 4 h after addition of 2.0 mM IPTG, biotransformation at 30oC, and supply of 300 μM naringenin. Through the optimized co-culture fermentation system, trifolin was biosynthesized up to 67.3 mg/L. ? The Korean Society for Applied Biological Chemistry 2021.
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농업생명과학대학 (환경산림과학부)
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