TRPV1 activation induces cell death of TM3 mouse Leydig cellsopen accessTRPV1 activation induces cell death of TM3 mouse Leydig cells
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- TRPV1 activation induces cell death of TM3 mouse Leydig cells
- 김은진; Dang Long Cao; Nyiramana Marie Merci; Siregar Adrian S.; 우민석; 김창운; 강다원
- Issue Date
- 사단법인 한국동물생명공학회
- apoptosis; Leydig cells; mice; testes; TRPV1
- 한국동물생명공학회지, v.36, no.3, pp.145 - 153
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- The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death.
Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicininduced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.
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- College of Medicine > Department of Medicine > Journal Articles
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