The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell lineThe in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
- Other Titles
- The in vitro effects of synthetic complement peptide C3a during Brucella abortus 544 infection in a murine professional phagocyte RAW264.7 cell line
- Authors
- Alisha Wehdnesday Bernardo Reyes; 김희진; TRAN XUAN NGOC HUY; Trang Thi Nguyen; 민원기; 김현진; 이후장; 김석
- Issue Date
- 2021
- Publisher
- 한국예방수의학회
- Keywords
- Brucella abortus; C3a; internalization; nitrite; RAW264.7
- Citation
- 예방수의학회지, v.45, no.4, pp 188 - 193
- Pages
- 6
- Indexed
- KCI
- Journal Title
- 예방수의학회지
- Volume
- 45
- Number
- 4
- Start Page
- 188
- End Page
- 193
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/4769
- ISSN
- 2287-7991
2287-8009
- Abstract
- We investigated the effect of a synthetic complement peptide C3a on the outcome of Brucella abortus 544 infection in a murine macrophage cell line RAW264.7 cell. First, we determined the highest non-cytotoxic concentration of the peptide in the cell line. We also found that the peptide significantly increased the growth of the bacteria at 8 and 24 h. Although the number of bacterial CFU was also elevated at 48 and 72 h, the increases were not significant as compared to controls. We further investigated the effect of C3a peptide on the growth of Brucella by pre-incubating the peptide at various temperatures and found that the effect was reversed at 24 h post-incubation suggesting that incubation of peptide at high temperatures including 65°C or 95°C could inactivate its action. This also could indicate the beneficial effect of high temperature during infection. Although several studies reported the inhibitory effect of different antimicrobial peptides including C3a, the present study preliminarily revealed that it had no positive contribution on the control of B. abortus 544 infection in vitro and indirectly to its receptor, CD88, which belongs to GPCR. Moreover, the encouraged further exploration of the effect of other similar peptides would be performed for the purpose of finding Brucella-host cell interaction for the control of disease progression.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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