Immunization With a Combination of Four Recombinant Brucella abortus Proteins Omp16, Omp19, Omp28, and L7/L12 Induces T Helper 1 Immune Response Against Virulent B. abortus 544 Infection in BALB/c Miceopen access
- Authors
- Huy Tran Xuan Ngoc; Nguyen Trang Thi; Reyes, Alisha Wehdnesday Bernardo; Vu, Son Hai; Min, WonGi; Lee, Hu Jang; Lee, John Hwa; Kim, Suk
- Issue Date
- 20-Jan-2021
- Publisher
- FRONTIERS MEDIA SA
- Keywords
- Brucella abortus; combined subunit vaccine; T helper 1 T cell; humoral immunity; macrophages
- Citation
- FRONTIERS IN VETERINARY SCIENCE, v.7
- Indexed
- SCIE
SCOPUS
- Journal Title
- FRONTIERS IN VETERINARY SCIENCE
- Volume
- 7
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/4220
- DOI
- 10.3389/fvets.2020.577026
- ISSN
- 2297-1769
- Abstract
- Protective efficiency of a combination of four recombinant Brucella abortus (B. abortus) proteins, namely outer membrane protein (Omp) 16, Omp19, Omp28, and 50S ribosomal protein L7/L12 was evaluated as a combined subunit vaccine (CSV) against B. abortus infection in RAW 264.7 cell line and murine model. The immunoreactivity of these four recombinant proteins as well as pCold-TF vector reacted with Brucella-positive serum individually, but not with Brucella-negative serum by immunoblotting assay. CSV-treated RAW 264.7 cells significantly induced production of IFN-gamma and IL-12 while decreased IL-10 production at the late stage of infection compared to PBS-treated control cells. In addition, the enhancement of nitric oxide production together with cytokines secretion profile in CSV-treated cells proved that CSV notably activated bactericidal mechanisms in macrophages. Consistently, mice immunized with CSV strongly elicited production of pro-inflammatory cytokines TNF-alpha, IL-6 and MCP-1 compared to PBS control group. Moreover, the concentration of IFN-gamma was >IL-10 and titers of IgG2a were also heightened compared to IgG1 in CSV-immunized mice which suggest that CSV induced predominantly T helper 1 T cell. These results suggest that the CSV used in the present study is a potential candidate as a preventive therapy against brucellosis.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.