Downregulation of swpa4 peroxidase expression in transgenic sweetpotato plants decreases abiotic stress tolerance and reduces stress-related peroxidase expression

Abstract

Class III peroxidases are involved in plant responses to various environmental conditions. Peroxidase production is triggered by various stimuli and activates stress-related signaling pathways to help plants cope with abiotic and biotic stresses. Recent evidence suggests that the interplay between diverse peroxidases plays a critical role in regulating stress responses. However, the underlying molecular mechanisms remain poorly understood. Previous research showed that transgenic sweetpotato overexpressing the swpa4 peroxidase gene exhibited increased tolerance to oxidative stress and high salinity. In this study, loss of function of the swpa4 peroxidase gene in sweetpotato was assessed in transgenic plants with constitutive RNAi-mediated suppression of the swpa4 gene. Compared with control plants, leaf discs from RNAi-suppressed lines exhibited increased damage following treatment with the oxidative stressors hydrogen peroxide and methyl viologen. Transgenic plants also showed reduced resistance to high salinity conditions compared with control plants. Repression of swpa4 also led to reduced expression of the endogenous stress-related peroxidase genes swpa1, swpa2, and swpn1. These findings suggest that swpa4 regulates signaling pathways involved in the regulation of peroxidase metabolism during the stress response in sweetpotato.