Optimizing the Saccharification of Pretreated Wood Biomass using Crude Enzyme from Acanthophysium Sp. KMF001open access
- Park, Sohyun; Yang, Jae-Kyung; Kim, Young-Kyoon; Yun, Jeonghee; Kim, Yeong-suk
- Issue Date
- NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI
- Cellulase; Optimization; Saccharification; stream-explosion; Acanthophysium sp. KMF001
- BIORESOURCES, v.16, no.1, pp.1207 - 1229
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- This study was conducted on crude enzyme from the novel strain Acanthophysium sp. KMF001 using a mediator (surfactant). The surfactant was applied to the steam-exploded pretreated domestic wood biomass, and response surface methodology (RSM) was conducted to determine the optimum conditions for saccharification using the optimum substrate concentration, enzyme concentration, and surfactant concentration. Steam-explosion of Korean oak (25 kgf/cm(2)) for 7 min showed a maximum-predicted saccharification of approximately 99.9% at 7.0% substrate concentration, 37.5 FPU (filter paper units) enzyme concentration, and 475.8 mg/g-glucan surfactant (polysorbate 80) concentration. Steam-explosion of red pine (25 kgf/cm(2)) for 7 min revealed a maximum prediction saccharification rate of approximately 58.7% at 6.5% substrate concentration, 36.3 FPU enzyme concentration, and 330.3 mg/g-glucan surfactant (polysorbate 80) concentration. The extents of saccharification of Korean oak (99.9%) and pine (58.7%) demonstrated the high applicability of the crude enzyme from Acanthophysium sp. KMF001.
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- 농업생명과학대학 > Department of Environmental Materials Science > Journal Articles
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