CRISPR/Cas-based precision genome editing via microhomology-mediated end joiningopen access
- Van Vu, Tien; Thi Hai Doan, Duong; Kim, Jihae; Sung, Yeon Woo; Thi Tran, Mil; Song, Young Jong; Das, Swati; Kim, Jae-Yean
- Issue Date
- MMEJ; precision gene editing; microhomology; CRISPR; cas; DNA repair; PITCh
- PLANT BIOTECHNOLOGY JOURNAL, v.19, no.2, pp.230 - 239
- Journal Title
- PLANT BIOTECHNOLOGY JOURNAL
- Start Page
- End Page
- Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide-directed mutagenesis (ODM), base editing, prime editing and especially homologous recombination (HR)-based gene targeting. With the advent of CRISPR/Cas for the targeted generation of DNA breaks (single-stranded breaks (SSBs) or double-stranded breaks (DSBs)), a substantial advancement in HR-mediated precise editing frequencies has been achieved. Nonetheless, further research needs to be performed for commercially viable applications of precise genome editing; hence, an alternative innovative method for genome editing may be required. Within this scope, we summarize recent progress regarding precision genome editing mediated by microhomology-mediated end joining (MMEJ) and discuss their potential applications in crop improvement.
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