Endothelial Cells Differentiated from Porcine Epiblast Stem Cells
- Jeon, Soo-Been; Seo, Bo-Gyeong; Baek, Sang-Ki; Lee, Hyeon-Geun; Shin, Joon-Hong; Lee, In-Won; Kim, Hyo-Jin; Moon, Sun Young; Shin, Keum-Chul; Choi, Jung-Woo; Kim, Tae-Suk; Lee, Joon-Hee; Hwangbo, Cheol
- Issue Date
- MARY ANN LIEBERT, INC
- porcine; epiblast stem cells; pluripotency; in vitro differentiation; endothelial cells
- CELLULAR REPROGRAMMING, v.23, no.2, pp.89 - 98
- Journal Title
- CELLULAR REPROGRAMMING
- Start Page
- End Page
- Pluripotent stem cells (PSCs) have the ability of self-renewal that can retain the characteristics of the mother cell, and of pluripotency that can differentiate into several body types. PSCs typically include embryonic stem cells (ESCs) derived from the inner cell mass of the preimplantation embryo, and epiblast stem cells (EpiSCs) derived from the epiblast of postimplantation embryo. Although PSCs are able to be used by differentiation into endothelial cells as a potential treatment for vascular diseases, human ESCs and induced PSCs (iPSCs) are followed by ethical and safety issues. Pigs are anatomically and physiologically similar to humans. Therefore, the goal of this study was to establish an efficient protocol that differentiates porcine EpiSCs (pEpiSCs) into the endothelial cells for applying the treatment of human vascular diseases. As a result, alkaline phosphatase (AP)-negative (-) pEpiSCs cultured in endothelial cell growth basal medium-2 (EBM-2) differentiation medium in association with 50 ng/mL of vascular endothelial growth factor (VEGF) for 8 days were changed morphologically like the feature of endothelial cells, and expression of pluripotency-associated markers (OCT-3/4, NANOG, SOX2, and C-MYC) in porcine differentiated cells was significantly decreased (p < 0.05). Additionally, when pEpiSCs were cultured in EBM-2 + 50 ng/mL of VEGF, porcine differentiated cells represented a common endothelial cell marker positive (CD31+) but monocytes and lymphocytes marker negative (CD45-). Therefore, these results indicated that pEpiSCs cultured in EBM-2 + 50 ng/mL of VEGF culture condition were efficiently differentiated into endothelial cells for the treatment of blood vessel diseases.
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- 농업생명과학대학 > 동물생명융합학부 > Journal Articles
- 농업생명과학대학 > 환경산림과학부 > Journal Articles
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