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Cited 6 time in webofscience Cited 6 time in scopus
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Regulatory molecule cAMP changes cell fitness of the engineered Escherichia coli for terpenoids production

Authors
Jeong, Seong-HeePark, Ji-BinWang, YanKim, Gye-HwanZhang, GaochuanWei, GongyuanWang, ChonglongKim, Seon-Won
Issue Date
May-2021
Publisher
Academic Press
Keywords
Cell fitness; cAMP; Terpenoids; Adenylate cyclase; Metabolic engineering
Citation
Metabolic Engineering, v.65, pp 178 - 184
Pages
7
Indexed
SCIE
SCOPUS
Journal Title
Metabolic Engineering
Volume
65
Start Page
178
End Page
184
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/3765
DOI
10.1016/j.ymben.2020.11.009
ISSN
1096-7176
1096-7184
Abstract
Terpenoids are a class of natural compounds with many important functions and applications. They are synthesized from a long synthetic pathway of isoprenyl unit coupling with the myriads of terpene synthases. Owing to the catalytic divergence of terpenoids synthesis, microbial production of terpenoids is compromised to the complexity of pathway engineering and suffers from the metabolic engineering burden. In this work, the adaptive Escherichia coli HP variant exhibited a general cell fitness in terpenoid synthesis. Especially, it could yield taxadiene of 193.2 mg/L in a test tube culture, which is a five-fold increase over the production in the wild type E. coli DH5?. Mutational analyses indicated that IS10 insertion in adenylate cyclase CyaA (CyaAHP) resulted in lowering intracellular cyclic AMP (cAMP), which could regulate its receptor protein CRP to rewire cell metabolism and contributed to the improved cell fitness. Our results suggested a way to manipulate cell fitness for terpenoids production and other products.
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