Development of a quantitative PCR primers and probe for environmental DNA detection of Pacific herring Clupea pallasii
- Gwak, Woo-Seok; Kouji, Nakayama
- Issue Date
- Pacific herring; eDNA; Quantitative PCR; Minor groove binding (MGB) probe
- CONSERVATION GENETICS RESOURCES, v.13, no.3, pp.337 - 339
- Journal Title
- CONSERVATION GENETICS RESOURCES
- Start Page
- End Page
- An eDNA assay for the detection of Pacific herring (Clupea pallasii) in water samples was developed using quantitative PCR (qPCR) technology. Species-specific primers and a minor groove binding (MGB) probe were designed based on the mitochondrial cytochrome b gene sequence. Upon conducting a cross-species test, the target species DNA was not only detected at low concentrations but also with high specificity. The assay was validated using field-collected water samples from aquatic environments known to be inhabited by Pacific herring and tested positive in samples from Pacific herring-inhabited sites. Our in silico, in vitro, and in situ results highlight the sensitivity of our proposed eDNA-based procedure for the detection of C. pallasii DNA, and could provide a non-invasive and non-destructive approach to detect this species and characterize its distribution in the Jinhaeman Bay, Korea.
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- 해양과학대학 > 양식생명과학과 > Journal Articles
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