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Cited 13 time in webofscience Cited 13 time in scopus
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N-acetylglucosaminyltransferase II Is Involved in Plant Growth and Development Under Stress Conditionsopen access

Authors
Yoo, Jae YongKo, Ki SeongBich Ngoc VuLee, Young EunYoon, Seok HanThao Thi PhamKim, Ji-YeonLim, Jae-MinKang, Yang JaeHong, Jong ChanLee, Kyun Oh
Issue Date
2-Nov-2021
Publisher
FRONTIERS MEDIA SA
Keywords
GnTII; N-glycan; structural diversity; functional diversity; plant development; stress response
Citation
FRONTIERS IN PLANT SCIENCE, v.12
Indexed
SCIE
SCOPUS
Journal Title
FRONTIERS IN PLANT SCIENCE
Volume
12
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/3011
DOI
10.3389/fpls.2021.761064
ISSN
1664-462X
Abstract
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [EC 2.4.1.143, N-acetylglucosaminyltransferase II (GnTII)] catalyzes the transfer of N-acetylglucosamine (GlcNAc) residue from the nucleotide sugar donor UDP-GlcNAc to the alpha 1,6-mannose residue of the di-antennary N-glycan acceptor GlcNAc(Xyl)Man(3)(Fuc)GlcNAc(2) in the Golgi apparatus. Although the formation of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan is known to be associated with GnTII activity in Arabidopsis thaliana, its physiological significance is still not fully understood in plants. To address the physiological importance of the GlcNAc(2)(Xyl)Man(3)(Fuc)GlcNAc(2) N-glycan, we examined the phenotypic effects of loss-of-function mutations in GnTII in the presence and absence of stress, and responsiveness to phytohormones. Prolonged stress induced by tunicamycin (TM) or sodium chloride (NaCl) treatment increased GnTII expression in wild-type Arabidopsis (ecotype Col-0) but caused severe developmental damage in GnTII loss-of-function mutants (gnt2-1 and gnt2-2). The absence of the 6-arm GlcNAc residue in the N-glycans in gnt2-1 facilitated the TM-induced unfolded protein response, accelerated dark-induced leaf senescence, and reduced cytokinin signaling, as well as susceptibility to cytokinin-induced root growth inhibition. Furthermore, gnt2-1 and gnt2-2 seedlings exhibited enhanced N-1-naphthylphthalamic acid-induced inhibition of tropic growth and development. Thus, GnTII's promotion of the 6-arm GlcNAc addition to N-glycans is important for plant growth and development under stress conditions, possibly via affecting glycoprotein folding and/or distribution.
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