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Inhibition of Autophagy Promotes Hemistepsin A-Induced Apoptosis via Reactive Oxygen Species-Mediated AMPK-Dependent Signaling in Human Prostate Cancer Cellsopen access

Authors
Kim, Kwang-YounYun, Un-JungYeom, Seung-HeeKim, Sang-ChanLee, Hu-JangAhn, Soon-CheolPark, Kwang-IlKim, Young-Woo
Issue Date
Dec-2021
Publisher
MDPI
Keywords
Hemistepsin A; AMPK; ROS; autophagy; apoptosis; prostate cancer
Citation
BIOMOLECULES, v.11, no.12
Indexed
SCIE
SCOPUS
Journal Title
BIOMOLECULES
Volume
11
Number
12
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/2943
DOI
10.3390/biom11121806
Abstract
Chemotherapy is an essential strategy for cancer treatment. On the other hand, consistent exposure to chemotherapeutic drugs induces chemo-resistance in cancer cells through a variety of mechanisms. Therefore, it is important to develop a new drug inhibiting chemo-resistance. Although hemistepsin A (HsA) is known to have anti-tumor effects, the molecular mechanisms of HsA-mediated cell death are unclear. Accordingly, this study examined whether HsA could induce apoptosis in aggressive prostate cancer cells, along with its underlying mechanism. Using HsA on two prostate cancer cell lines, PC-3 and LNCaP cells, the cell analysis and in vivo xenograft model were assayed. In this study, HsA induced apoptosis and autophagy in PC-3 cells. HsA-mediated ROS production attenuated HsA-induced apoptosis and autophagy after treatment with N-acetyl-L-cysteine (NAC), a ROS scavenger. Moreover, autophagy inhibition by 3-MA or CQ is involved in accelerating the apoptosis induced by HsA. Furthermore, we showed the anti-tumor effects of HsA in mice, as assessed by the reduced growth of the xenografted tumors. In conclusion, HsA induced apoptosis and ROS generation, which were blocked by protective autophagy signaling.
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