Detailed Information

Cited 1 time in webofscience Cited 1 time in scopus
Metadata Downloads

Effect of deregulation of repressor-specific carbon catabolite repression on carbon source consumption in Escherichia coliopen access

Authors
Seong, Hyeon JeongJang, Yu-Sin
Issue Date
Dec-2021
Publisher
SPRINGER SINGAPORE PTE LTD
Keywords
Escherichia coli; Carbon catabolite repression; Glucose; Galactose; Galactose operon
Citation
APPLIED BIOLOGICAL CHEMISTRY, v.64, no.1
Indexed
SCIE
SCOPUS
KCI
Journal Title
APPLIED BIOLOGICAL CHEMISTRY
Volume
64
Number
1
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/2920
DOI
10.1186/s13765-021-00627-0
ISSN
2468-0834
Abstract
Escherichia coli has been used as a host to construct the cell factory for biobased production of chemicals from renewable feedstocks. Because galactose is found in marine biomass as a major component, the strategy for galactose utilization in E. coli has been gained more attention. Although galactose and glucose co-fermentation has been reported using the engineered E. coli strain, few reports have covered fermentation supplemented with galactose as a sole carbon source in the mutant lacking the repressor-specific carbon catabolite repression (CCR). Here, we report the effects of the deregulation of the repressor-specific CCR (galR(-) and galS(-)) in fermentation supplemented with galactose as a sole carbon source, using the engineered E. coli strains. In the fermentation using the galR(-) and galS(-) double mutant (GR2 strain), an increase of rates in sugar consumption and cell growth was observed compared to the parent strain. In the glucose fermentation, wild-type W3110 and its mutant GR2 and GR2PZ (galR(-), galS(-), pfkA(-), and zwf(-)) consumed sugar at a higher rate than those values obtained from galactose fermentation. However, the GR2P strain (galR(-), galS(-), and pfkA(-)) showed no difference between fermentations using glucose and galactose as a sole carbon source. This study provides essential information for galactose fermentation using the CCR-deregulated E. coli strains.
Files in This Item
There are no files associated with this item.
Appears in
Collections
ETC > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jang, Yu Sin photo

Jang, Yu Sin
대학원 (응용생명과학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE