Biodegradable properties of organophosphorus insecticides by the potential probiotic Lactobacillus plantarum WCP931 with a degrading gene (opdC)open access
- Lee, Jin Hwan; Lee, Hee Yul; Cho, Du Yong; Kim, Min Ju; Jung, Jea Gack; Jeong, Eun Hye; Haque, Md Azizul; Cho, Kye Man
- Issue Date
- SPRINGER SINGAPORE PTE LTD
- Organophosphorus insecticides; Kimchi; Lactobacillus plantarum WCP931; OpdC gene; Biodegradation
- APPLIED BIOLOGICAL CHEMISTRY, v.64, no.1
- Journal Title
- APPLIED BIOLOGICAL CHEMISTRY
- An organophosphorus (OP) insecticide-mineralizing strain, Lactobacillus plantarum WCP931, harboring a new OP hydrolase (opdC) gene, was isolated during kimchi (Korean traditional food) fermentation. Strain WCP931 exhibited a significant survival rate of 51 to 96% under artificial gastric acid conditions at pH 2 to 3 after 3 h. The opdC gene, consisting of 831 bp encoding 276 amino acids, was cloned from strain WCP907. Recombinant Escherischia coli harboring the opdC gene depleted 77% chlorpyrifos (CP) in M9 minimal medium after 6 days of incubation. The OpdC enzyme represents a novel member of the GHSQG family of esterolytic enzymes or a new Opd group. The OpdC molecular mass was estimated to be approximately 31 kDa by SDS-PAGE and showed maximum activity at pH 6 and 35 degrees C. The mutated OpdC (Ser116 -> Ala116) enzyme had no activity towards OP insecticides and rho-nitrophenol-beta-butyrate. Importantly, the relative activity of OpdC protein against chlorpyrifos, coumafos, diazinon, fenamifos, methyl parathion, and parathion was higher than that against cadosafos, dyfonate, and ethoprofos insecticides. These results suggested the involvement of OpdC in the biodegradation of OP insecticide-contaminated cabbage during fermentation. The new OpdC enzyme expands the heterogeneity of the lactic acid bacterial Opd enzyme group in nature.
- Files in This Item
- There are no files associated with this item.
- Appears in
- 농업생명과학대학 > 식품공학부 > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.