Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cellsopen access
- Authors
- Jin, Guang-Zhen; Yin, Xi-Jun; Yu, Xian-Feng; Cho, Su-Jin; Lee, Hyo-Sang; Lee, Hyo-Jong; Kong, Il-Keun
- Issue Date
- Dec-2007
- Publisher
- KOREAN SOC VETERINARY SCIENCE
- Keywords
- co-culture; feline bone marrow; mesenchymal stem cell; rat PC12 cell; tyrosine hydroxylase
- Citation
- JOURNAL OF VETERINARY SCIENCE, v.8, no.4, pp.377 - 382
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF VETERINARY SCIENCE
- Volume
- 8
- Number
- 4
- Start Page
- 377
- End Page
- 382
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/28228
- DOI
- 10.4142/jvs.2007.8.4.377
- ISSN
- 1229-845X
- Abstract
- Mesenchymal stem cells (MSCs) secrete a variety of neuroregulatory molecules, such as nerve growth factor, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor, which upregulate tyrosine hydroxylase (TH) gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson's disease (PD). The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups: an MSC group, a PC12 cell group, and the combined MSC + PC12 cell group (the co-culture group). All cells were cultured in DMEM-HG medium supplemented with 10% fetal bovine serum for three days. Thereafter, the cells were examined using western blot analysis and immunocytochemistry. In western blots, the co-culture group demonstrated a stronger signal at 60 kDa than the PC 12 cell group (p < 0.001). TH was not expressed in the MSC group, either in western blot or immunocytochemistry. Thus, the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neurodegenerative disease process.
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