Iridin abrogates LPS-induced inflammation in L6 skeletal muscle cells by inhibiting NF-kappa B and MAPK signaling pathway

Abstract

Background Iridin is a glycoside form of flavonoids isolated from Belamcanda chinensis, and Iris florentina has numerous biological properties, including antitumor, antiproliferative, anti-inflammatory and antioxidant properties. Inflammatory diseases are an essential health concern and have a growing incidence worldwide, so developing safe drugs remains an important objective. Objective In the present study, we evaluated iridin's anti-inflammatory effect on LPS-stimulated skeletal muscle cells (L6). The anti-inflammatory action of iridin elucidated through NF-kappa B (nuclear factor-kappa B) and MAPK (mitogen-activated protein kinases) pathway in LPS-stimulated L6 cells. Results Determination of cytotoxicity was examined by MTT assay. The expression level of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) was evaluated by western blot analysis. To elucidate the underlying mechanism, NF-kappa B and MAPKs proteins were investigated by immunoblot. Iridin could decrease the phosphorylation of NF-kappa B and MAPK proteins in LPS-induced L6 cells. Conclusion The results showed that iridin inhibited the phosphorylation of NF-kappa B and MAPK proteins and inflammatory cytokines COX-2 and iNOS in LPS-induced L6 cells. Based on findings in this work, iridin possesses anti-inflammatory action on L6 cells and could be considered for an attractive candidate for anti-inflammation.